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A spinning disk confocal microscope simultaneously illuminates of many excitation light areas and you can detects the new produced fluorescence from for each place inside synchronous. A spinning confocal computer goes through excitation places when you are related pinholes to your the brand new advanced imaging plane reduce the of attention fluorescence. The brand new spatial distribution of the fluorescence signal will then be sensed by the a camera (Figure 2). While the found from the plan of pinhole number in the Shape 2, the length anywhere between pinholes on the confocal computer are adequate to avoid laws crosstalk anywhere between pinholes. In addition, it realizes fast imaging as fast as 200 fps by using rotating computer confocaⅼ fluorescence microscopy. However, the real resolution can be just like compared to a widefield fluorescence microscope while the high-volume signals are weakened.

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